Technical workshops

Overview

In the 36th IMGC, Technical workshops are held on the morning of the first day, March 28 (Tue).

Planned workshops are:

Workshop 1: Engineered Allele Validation Workshop at room 304
(Organized by Lydia Teboul, Mary Lyon Centre, MRC Harwell)
Workshop 2: Practical aspects of designing and developing genetically engineered animal models at room 201A
(Organized by Channabasavaiah Gurumurthy, University of Nebraska Medical Center)
Workshop 3: The Alliance of Genome Resources and MGI – what’s new and in progress at room 201B
(Organized by Cynthia L. Smith (Mouse Genome Informatics, The Jackson Laboratory)

Workshop 1 (at room 304): Engineered Allele Validation Workshop

MRC Harwell and PHENOMIN-ICS are co-hosting at IMGC 2023 Tsukuba, Japan, a workshop to learn more about the principles of analysis and validation of Genetically Altered animals.

Topics covered:

Principles of allele validation
Validation of randomly integrated transgenes: Genotyping, copy counting, mapping
Validation of classically targeted alleles: PCR based methods, Southern, distal loxP
Validation of GE alleles: Strategies for analysis, G0/G1, Sequencing and copy counting
Functional validation: Gene products, cre activity

The Workshop will start at 9 am March 28th and last 4 hours. This will be an interactive in-person event with capacity of 25 delegates to facilitate discussions.
Cost of the training will be 60 Euros.

Workshop 2 (at room 201A): Practical aspects of designing and developing genetically engineered animal models.

Genetically engineered animal models are widely used in basic and translational research. This workshop aims to introduce concepts of designing and generating animal models. The workshop is intended for entry level researchers but at the same time it will be flexible enough to accommodate researchers with prior experience in the transgenic technologies. The workshop will cover widely adapted CRISPR methods including latest advances in the field, with an intention to provide a comprehensive training on the subject.

The workshop will cover:

Introduction to traditional and CRISPR based methods of generating animal models
Overview and comprehensive discussion of current methods of generating animal models
Discussion of methods of identifying on target genomic modifications and ruling out off target editing events
Review of case studies: one example each of small insertion, large insertion, and conditional knockout mouse model designs (from published papers)
Designing animal models of participants’ projects: i.e., real-life examples

The workshop will be held in-person and free of charge, but registration is mandatory.

Methods, protocols, review articles or model design example papers relevant to the workshop:

Workshop 3 (at room 201B): The Alliance of Genome Resources and MGI – what’s new and in progress

Mouse Genome Informatics (http://www.informatics.jax.org/) is a free, highly curated resource providing searchable web and programmatic access to complete catalog of mouse genes and genome features, functional annotations, variation, gene expression data and a comprehensive index of mutant and knockout alleles, phenotypes and human disease models.

The Alliance of Genome Resources (https://www.alliancegenome.org/) is a consortium of 7 model organism databases (MODs, including MGI) and the Gene Ontology (GO) Consortium whose goal is to provide an integrated view of their free, highly curated data to all biologists, clinicians and other interested parties. The primary mission of the Alliance of Genome Resources (the Alliance) is to develop and maintain sustainable genome information resources that facilitate the use of diverse model organisms in understanding the genetic and genomic basis of human biology, health and disease. This understanding is fundamental for advancing genome biology research and for translating model organism and human genome data into clinical utility.

This workshop is designed to include short seminar introductions to features and tools of both resources, highlighting new data and functionality of these complementary resources. We will review examples of complex queries and the different ways to retrieve data relevant to research questions. Attendees are also encouraged to bring their own research-specific questions for discussion.

This workshop is free of charge.

MGI team plans to stream this workshop for those attending virtually.